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Yeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required
2 at 25°C Suspend 30 gram powdered medium in 1 litre distilled water
Cold Spring harbour
the maltose-fermenting strains will change the Bromcresol purple indicator into yellow
7 gram powdered medium in 1 litre distilled water
HEPES Size:1000g Yeast Nitrogen Base is basedC8H18N2O4S=238. 3 Specifications HEPES >99. 5% Loss on Drying (105 C 4hr) <0. 5% Water Content (By KF) <0. 5% Infrared Complies pH 1% in water 4. 8 5. 6 (25C) Solubility 1M in water Clear and colourless solution Residue on ignition <0. 1% A260, 1M in water <0. 045 A280, 1M in water <0. 035 A420, 1M in water <0. 20 Heavy Metals <5 ppm pKa at 20C 7. 35 7. 69 Store dry at room temperature Soluble in water CAS 7365 45 9
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